Ben Proud successfully defended his men’s 50 metres freestyle title but could not double up as England were pipped to another swimming gold by Australia in the 4 x 100m medley.

Proud set Games records in the freestyle heat and in the semi-final and clocked 21.35 seconds to take gold ahead of South Africa’s Bradley Tandy.

In the final race of the night Proud was bidding to bring England home but Kyle Chambers overhauled him in the final stroke.

Australia won in a Games record three minutes 31.04 seconds, with England’s Luke Greenbank, Adam Peaty, James Guy and Proud just 0.09secs back

Holly Hibbott took silver and Ellie Faulkner bronze as Australia’s Ariarne Titmus won the women’s 400m freestyle in a Games record of four minutes 00.93 seconds.

Scotland’s Duncan Scott got a sixth medal of the games with silver in the men’s 200 metres individual medley final.

Georgia Davies took women’s 50m backstroke bronze behind Australia’s Emily Seebohm, and there was more to come for Wales as Dan Jervis, the part-time painter and decorator, took silver in the men’s 1500m freestyle.

Davies, Chloe Tutton, Alys Thomas and Kathryn Greenslade picked up bronze in the women’s 4 x 100m medley.

It turned into a night of frustration at the athletics as reigning world champion Andy Pozzi of England could only finish sixth in a 110m hurdles final which was won by Jamaica’s Ronald Levy.

Sophie Hitchon was then eliminated from the hammer throw final after fouling three times.

Melissa Courtney picked up bronze for Wales in the women’s 1500m, won by Caster Semenya.

England won silver and bronze in the road cycling time trials despite the embarrassment of an administrative error which cost Melissa Lowther the opportunity to compete at all.

Team England admitted they failed to properly file Lowther’s entry, ruling her out of an event she has focused on throughout the season so far.

Of those who did race, Harry Tanfield took silver in the men’s time trial while Hayley Simmonds collected bronze in the women’s event.

At the shooting, David Phelps took gold in the 50m Rifle Prone, a seventh gold medal of the Games for Wales as they overtook their tally from Glasgow four years ago.

England’s Parag Patel and David Luckman won gold in a Queen’s Prize Pairs shooting Home Nations 1-2-3. Wales’ Chris Watson and Gareth Morris took silver and Scotland’s Alex Walker and Ian Shaw bronze.

Canadian Robert Pitcairn, who at 79 is the oldest athlete in the history of the Games, finished eighth with his partner Nicole Rossignol.

From the oldest athlete to the youngest – as 11-year-old Anna Hursey of Wales opened play in the table tennis singles with a 4-0 win over Halima Nambozo of Uganda, before losing by the same score to Malaysian Li Sian Alice Chang later in the day.

Birtley twin brothers Luke and Pat McCormack guaranteed at least boxing bronze medals with victories in their respective light-welterweight and welterweight categories.

There was Para Powerlifting silver for Louise Sugden in the women’s heavyweight final and bronze medals for Ali Jawad and Zoe Nelson in the men’s and women’s lightweight finals.

Sam Ward bagged a hat-trick as England came from 2-0 down to beat Wales 3-2 and book their place in the men’s hockey semi-finals.

And England triumphed over Scotland in the beach volleyball quarter-finals, with Chris Gregory and Jake Sheaf recording a 21-14 21-17 win over Robin Brodzski and Seain Cook.

Scotland set up a men’s basketball semi-final against hosts Australia with a 66-61 win over Nigeria, but England are out following a 97-79 loss to Canada.

England’s women advanced to the final four with a 62-40 win over Jamaica.